Spectral studies on the binding behavior of cationic dyes and surfactants with bacterial polysaccharide of Klebsiella K43 Abstract The Binding behavior of acidic capsular polysaccharide (SPS), isolated from Klebsiella serotype K43 with oppositely charged dyes
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چکیده
The Binding behavior of acidic capsular polysaccharide (SPS), isolated from Klebsiella serotype K43 with oppositely charged dyes and surfactants have been studied by absorbance and emission spectroscopic studies. Each repeating unit of the SPS consists of three D-mannose, one D-galactose and one D-glucuronic acid residue. The anionic polymer exhibited chromtropic character and induced strong metachromasy in the cationic dye, pinacyanol chloride (PCYN) through the formation of a 1:1 polymer/dye complex. Evaluation of thermodynamic parameters, viz., standard changes in free energy (∆G 0 ), enthalpy (∆H 0 ) and entropy (∆S 0 ), for the formation of dye-polymer complex and studies on the effect of different co-solvents were also evaluated to shed light on the binding nature as well as the extent of stability of the dye-polymer complex. Fluorescence of the cationic dye acridine orange (AO) was quenched with the progressive addition of SPS, which was found to be of Stern-Volmer type. Cationic surfactants in their pure form as well as in mixed state with nonionic surfactant (Tween 20), replaced the dye bound to the polymer matrices; thus the original band intensities of the dyes could be reverted. Such the studies revealed that both electrostatics as well as hydrophobic forces is operative within the dye-polymer as well as surfactant-polymer aggregates. Published in J. Dispersion Sci. Technol. 31 (2010) 1447-1455
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